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digestion buffer (Miltenyi Biotec)

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Miltenyi Biotec digestion buffer
Digestion Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+tissue+dissociation+kit/Adipose+Tissue+Dissociation+Kit%2C+mouse+and+rat/10__1007_slash_s12195___026___00925___x-285-17-19
Average 95 stars, based on 71 article reviews

digestion buffer - by Bioz Stars, 2026-10

95/100 stars

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Article Title: Chromobox 2 inhibition: A novel activity of alisertib, an Aurora A kinase inhibitor
Article Snippet: Briefly, minced tumors were transferred to gentleMACs C Tubes (Miltenyi Biotec, Cat #130-093-237) containing RPMI with Miltenyi enzymes A, R, and D. GentleMACs tubes with tumors and enzyme mixture were then moved to gentleMACs Octo Dissociator and incubated at 37°C for 40 minutes with continuous rotation.

Article Title: DNA methylation in small cell lung cancer defines distinct disease subtypes and correlates with high expression of EZH2
Article Snippet: PDXs were propagated as previously described with the addition of a dissociation step using a gentleMACS automated dissociator and mouse tissue dissociation kit (Miltenyi) prior to resuspension in 50% matrigel in HBSS ( ).

Article Title: Decrypting cellular engagement and recruitment from spatially resolved transcriptomics
Article Snippet: After perfusion with PBS via the right ventricle, hearts were immediately harvested, minced, and enzymatically digested using a mouse tissue dissociation kit (Miltenyi Biotec Inc., Auburn, CA, USA, Cat# 130–096–730) at 37°C for 10 minutes in a rotating incubator.

Flow Cytometry:

Article Title: Comparative study of nanoparticle uptake and impact in murine lung, liver and kidney tissue slices.
Article Snippet: .. Tissue digestion and flow cytometry analysis The Mouse tissue dissociation kit from Miltenyi Biotec (Bergisch Gladbach, Germany) was used for the enzymatic digestion of murine lung slices. ..

Single Cell:

Article Title: Circulating mitochondrial DNA promotes M2 polarization of tumor associated macrophages and HCC resistance to sorafenib.
Article Snippet: .. Single cell suspensions were prepared from fresh mouse tumors by using the mouse tissue dissociation kit (Miltenyi, cat. 130-096-730) following the manufacturer’s instructions. ..

Article Title: Circulating mitochondrial DNA promotes M2 polarization of tumor associated macrophages and HCC resistance to sorafenib
Article Snippet: .. Single cell suspensions were prepared from fresh mouse tumors by using the mouse tissue dissociation kit (Miltenyi, cat. 130-096-730) following the manufacturer’s instructions. ..